Figure 5.Quantification of tumor-informed SVs of HGSOCs in liquid biopsy(A–D) Concentrations (copy numbers per ng sample input) of targeted SVs were measured by ddPCR (digital droplet PCR). Each column represents the mean value of 1 or 2 replicates due to sample limitations. Germline control WBC(white blood cell) and subject pre-treatment cfDNA (cell-free DNA) of each HGSOC, pooled healthy cfDNA and a NTC were used for all experiments. (A) In HGSOC#1, 4 SV targets (SV1, 3, 4, 5) were measured. (B) In HGSOC#2, 3 SV targets (SV1, 4, 6) were measured. (C) In HGSOC#3, subject post-surgery cfDNA was also used in ddPCR and 4 SV targets (SV1, 3, 4, 8) were measured. (D) In HGSOC#4, 4 SV targets (SV1, 2, 3, 5) were measured.
