Figure 3.Validation of SVs breakpoints-spanning primers/probe sets by real-time PCR(A–D) Real-time PCR results of targeted SVs. ΔCq (ΔCq = Cq.Target - Cq.GAPDH) were calculated and compared in HGSOC#1-4. Each dot represents the mean value of three duplicates. Germline control WBC (white blood cell) of each HGSOC and NTC (no template control) were used for all experiments. (A) In HGSOC#1, gDNA of multisite biopsies LOV (left ovary), ROV (right ovary), and RECT (rectus) were used for real-time PCR and 6 SV targets (SV1-6) were tested. (B) In HGSOC#2, gDNA of multisite biopsies OV (ovary), OM (omentum), and PERI (pericardium) were used for real-time PCR and 6 SV targets (SV1-6) were tested. (C) In HGSOC#3, gDNA of multisite biopsies LOV (left ovary), LPELV (left pelvis), and RABD (right abdominum) were used for real-time PCR and 8 SV targets (SV1-8) were tested. (D) In HGSOC#4, gDNA of biopsies OM (omentum) and PELVIS (pelvis) were used for real-time PCR and 9 SV targets (SV1-9) were tested.
