Figure 3.SB-216 significantly reduces mRNA expression of oncogenic tubulin subtypes βIII and βIVB and protein expression of βIIISB-216 reduces mRNA expression of TUBB3 and TUBB4B in (A) PDCL110 and (B) Panc-1 cells. (C) SB-216 reduces protein expression of TUBB3 and TUBB4B. PDCL110 and Panc-1 cells were treated with SB-216 (5 nM) or DMSO (control) for 48 hours. RNA was isolated and transcribed for cDNA preparation. qPCR was performed to determine mRNA expression. Actin was used as an internal control. Graphs represent relative fold change expression of each tubulin mRNA expression. (Values mean ± SEM, n = 8,4). PDCL110 and Panc-1 cells were treated with SB-216 (5 nM) or DMSO (control) for 48 hours. Protein expression was evaluated with western blot. GAPDH was used as the loading control (and is same from Figure 2A). Asterisks represent a significant reduction in mRNA expression (*p < 0.05). Panc1 TUBB3 protein blot was the same as BRD4 blot with the same loading control GAPDH as on Figure 2A.
